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dapi stock solution  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
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    Structured Review

    Thermo Fisher dapi stock solution
    Dapi Stock Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+stock+solution/dapi+stock+solution/pm40652833-199-13-10
    Average 90 stars, based on 1 article reviews
    dapi stock solution - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Modification:

    Article Title: Composable microfluidic spinning platforms for facile production of biomimetic perfusable hydrogel microtubes.
    Article Snippet: Microtissues with specific structures and integrated vessels play a key role in maintaining organ functions.. To recapitulate the in vivo environment for tissue engineering and organ-on-a-chip purposes, it is essential to develop perfusable biomimetic microscaffolds.. We developed facile all-aqueous microfluidic approaches for producing perfusable hydrogel microtubes with diverse biomimetic sizes and shapes.

    Staining:

    Article Title: Pharmacokinetics of Pars Plana Intravitreal Injections versus Microcannula Suprachoroidal Injections of Bevacizumab in a Porcine Model
    Article Snippet: .. DAPI (4′,6-diamidino-2-phenylindole, dihydrochloride) staining of the retinal and RPE cell nuclei was performed by using a dilution of the DAPI stock solution (Molecular Probes, Eugene OR) to 300 nM in phosphate-buffered saline (PBS). ..

    Article Title: HRK downregulation and augmented BCL-xL binding to BAK confer apoptotic protection to therapy-induced senescent melanoma cells.
    Article Snippet: Secondary antibody incubation was carried out by incubating cells with anti-Mouse IgG (H+ L), SuperclonalTM Recombinant Secondary Antibody Alexa FluorTM 488 (A28175, Thermo Fisher) and anti-Rabbit IgG (H+ L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 (A21428, Thermo Fisher) for 45min in 0.02% saponin and 0.1% BSA solution at RT. .. For DAPI staining, cells were incubated with diluted DAPI stock solution (10374168, Thermo Fisher) to a final concentration of 300 nM in PBS for 5 min. .. Following staining, cells were washed three times with PBS, rinsed in water and mounted in Mowiol® 4–88 (17951–500, Polysciences, Warrington, PA, USA). siRNA-mediated gene knockdown For knockdown experiments, cells were transfected with HRK siRNA SMARTpool (77L-HUMAN-XX-0005, Horizon Discovery, Cambridge, UK) or non-targeting siRNA pool (77D-001810-10-7505, Horizon Discovery) using Lipofectamine® RNAiMax (13778-075, Thermo Fisher).

    Article Title: Double network microgels based on dextran and fibrin with tunable structural, mechanical, and degradation properties.
    Article Snippet: The samples were stained for 1 h using Phalloidin-iFluor 488 (abcam) stock solution (diluted 1:1000 in PBS, 1 mL) to stain the F-actin (green). .. After washing the sample with PBS (3×), DAPI (≥95 %, Thermo Fisher Scientific) stock solution at 300 μM was diluted 1:200 in PBS and added for 20 min to stain the nucleus of the cells (blue). ..

    Saline:

    Article Title: Pharmacokinetics of Pars Plana Intravitreal Injections versus Microcannula Suprachoroidal Injections of Bevacizumab in a Porcine Model
    Article Snippet: .. DAPI (4′,6-diamidino-2-phenylindole, dihydrochloride) staining of the retinal and RPE cell nuclei was performed by using a dilution of the DAPI stock solution (Molecular Probes, Eugene OR) to 300 nM in phosphate-buffered saline (PBS). ..

    Incubation:

    Article Title: HRK downregulation and augmented BCL-xL binding to BAK confer apoptotic protection to therapy-induced senescent melanoma cells.
    Article Snippet: Secondary antibody incubation was carried out by incubating cells with anti-Mouse IgG (H+ L), SuperclonalTM Recombinant Secondary Antibody Alexa FluorTM 488 (A28175, Thermo Fisher) and anti-Rabbit IgG (H+ L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 (A21428, Thermo Fisher) for 45min in 0.02% saponin and 0.1% BSA solution at RT. .. For DAPI staining, cells were incubated with diluted DAPI stock solution (10374168, Thermo Fisher) to a final concentration of 300 nM in PBS for 5 min. .. Following staining, cells were washed three times with PBS, rinsed in water and mounted in Mowiol® 4–88 (17951–500, Polysciences, Warrington, PA, USA). siRNA-mediated gene knockdown For knockdown experiments, cells were transfected with HRK siRNA SMARTpool (77L-HUMAN-XX-0005, Horizon Discovery, Cambridge, UK) or non-targeting siRNA pool (77D-001810-10-7505, Horizon Discovery) using Lipofectamine® RNAiMax (13778-075, Thermo Fisher).

    Concentration Assay:

    Article Title: HRK downregulation and augmented BCL-xL binding to BAK confer apoptotic protection to therapy-induced senescent melanoma cells.
    Article Snippet: Secondary antibody incubation was carried out by incubating cells with anti-Mouse IgG (H+ L), SuperclonalTM Recombinant Secondary Antibody Alexa FluorTM 488 (A28175, Thermo Fisher) and anti-Rabbit IgG (H+ L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 (A21428, Thermo Fisher) for 45min in 0.02% saponin and 0.1% BSA solution at RT. .. For DAPI staining, cells were incubated with diluted DAPI stock solution (10374168, Thermo Fisher) to a final concentration of 300 nM in PBS for 5 min. .. Following staining, cells were washed three times with PBS, rinsed in water and mounted in Mowiol® 4–88 (17951–500, Polysciences, Warrington, PA, USA). siRNA-mediated gene knockdown For knockdown experiments, cells were transfected with HRK siRNA SMARTpool (77L-HUMAN-XX-0005, Horizon Discovery, Cambridge, UK) or non-targeting siRNA pool (77D-001810-10-7505, Horizon Discovery) using Lipofectamine® RNAiMax (13778-075, Thermo Fisher).

    other:

    Article Title: Insights into the limitations of transient expression systems for the functional study of p53 acetylation site and oncogenic mutants.
    Article Snippet: Tumor suppressor protein p53 protects cells against malignant transformation mostly through transcriptional activation.. Lysine acetylation is required to mediate activation of p53.. The protein displays eight lysine residues and their evolutionary conservation argues for an essential role.



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